ARHGAP4 Polyclonal antibody

ARHGAP4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16697-1-AP

Synonyms

ARHGAP4, C1, KIAA0131, p115, RGC1, RhoGAP4



Tested Applications

Positive WB detected inHL-60 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16697-1-AP targets ARHGAP4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP4 fusion protein Ag10091
Full Name Rho GTPase activating protein 4
Calculated molecular weight 986 aa, 109 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC052303
Gene symbol ARHGAP4
Gene ID (NCBI) 393
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IHC protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IF protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IP protocol for ARHGAP4 antibody 16697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols