ARHGAP4 Recombinant antibody, PBS Only

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  WB analysis using 85648-2-RR (same clone as 85648-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85648-2-RR (ARHGAP4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85648-2-RR (same clone as 85648-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (85648-2-RR, Clone: 242995B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85648-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85648-2-RR against Human ARHGAP4 were performed. The affinity constant is 0.518 nM.

ARHGAP4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85648-2-PBS

Clone No.242995B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1

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Product Information

85648-2-PBS targets ARHGAP4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARHGAP4 fusion protein Ag10091
• Full Name: Rho GTPase activating protein 4
• Calculated Molecular Weight: 986 aa, 109 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC052303
• Gene Symbol: ARHGAP4
• Gene ID (NCBI): 393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98171
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARHGAP4 (Rho GTPase-Activating Protein 4), also named as KIAA0131, RGC1, RHOGAP4, is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating these signaling molecules. Rho GTPases are critical regulators of cytoskeletal dynamics, cell motility, and intracellular trafficking.