ARHGEF1 Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• ARHGEF1 Recombinant monoclonal antibody, PBS Only (88129-1-PBS)

ARHGEF1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 88129-1-PBS

Clone No.260491F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF

• 

  WB analysis using 88129-1-RR (same clone as 88129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88129-1-RR (ARHGEF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88129-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 88129-1-RR (same clone as 88129-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using ARHGEF1 antibody (88129-1-RR, Clone: 260491F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88129-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

88129-1-PBS targets ARHGEF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1905

  Product name: Recombinant human ARHGEF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC011726

  Sequence: MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEP

• Full Name: Rho guanine nucleotide exchange factor (GEF) 1
• Calculated Molecular Weight: 912 aa, 102 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC011726
• Gene Symbol: ARHGEF1
• Gene ID (NCBI): 9138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92888
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARHGEF1, also known as p115-RhoGEF, is a member of the Rho guanine nucleotide exchange factor (GEF) family, specifically activating the small GTPase RhoA by catalyzing the exchange of GDP for GTP. Structurally, it contains a Dbl homology (DH) domain responsible for GEF activity, a Pleckstrin homology (PH) domain that may regulate membrane association, and an RGS-like domain that interacts with the G-protein subunits Gα12/13. This protein is expressed ubiquitously, with particularly high expression in hematopoietic and lymphoid tissues, and plays a critical role in transducing signals from G protein-coupled receptors coupled to Gα12/13 to activate RhoA and its downstream effector ROCK, thereby regulating cytoskeletal dynamics, cell contraction, and migration. Notably, ARHGEF1 acts as a dual-specificity regulator, functioning as a GAP for Gα12/13 while serving as a GEF for RhoA. Its involvement in essential physiological processes, such as angiotensin II-induced vascular smooth muscle contraction, underscores its importance, while mutations in the ARHGEF1 gene are associated with immunodeficiency 62 and other potential pathologies.