ARHGEF10 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARHGEF10 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11112-1-AP

Synonyms

ARHGEF10, GEF10, KIAA0294

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Validation Data Gallery

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  IHC staining of human lung cancer using 11112-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11112-1-AP (ARHGEF10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11112-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11112-1-AP (ARHGEF10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

11112-1-AP targets ARHGEF10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGEF10 fusion protein Ag1605
• Full Name: Rho guanine nucleotide exchange factor (GEF) 10
• Calculated Molecular Weight: 147 kDa
• GenBank Accession Number: BC036809
• Gene Symbol: ARHGEF10
• Gene ID (NCBI): 9639
• RRID: AB_2059754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF10(Rho guanine nucleotide exchange factor 10), also named as KIAA0294, is a member of the family of Rho guanine nucleotide exchange factors (GEFs), which are implicated in neural morphogenesis and connectivity and regulate the activity of small Rho GTPases by catalyzing the exchange of bound GDP by GTP(PMID:14508709). The deduced 1,121-amino acid protein shares significant similarity with cell signaling proteins(PMID:9205841). Defects in ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV)(PMID:14508709). It has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB,IF	J Thromb Haemost; ARHGEF10 knockout inhibits platelet aggregation and protects mice from thrombus formation.; Authors - Dai-Hua Lu ; KO Validated
mouse	WB	Mol Autism; Impairment of social behaviors inArhgef10knockout mice.; Authors - Dai-Hua Lu ; KO Validated