ARHGEF11 Polyclonal antibody
ARHGEF11 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 30508-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, HuH-7 cells, MDA-MB-453 cells, mouse brain tissue |
Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30508-1-AP targets ARHGEF11 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ARHGEF11 fusion protein Ag33104 |
Full Name | Rho guanine nucleotide exchange factor (GEF) 11 |
Calculated Molecular Weight | 168 kDa |
Observed Molecular Weight | 200-220 kDa |
Gene Symbol | ARHGEF11 |
Gene ID (NCBI) | 9826 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).
Protocols
Product Specific Protocols | |
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WB protocol for ARHGEF11 antibody 30508-1-AP | Download protocol |
IHC protocol for ARHGEF11 antibody 30508-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |