ARHGEF7 Polyclonal antibody

ARHGEF7 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14092-1-AP

Synonyms

ARHGEF7, BETA PIX, COOL 1, COOL1, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PAK3BP, PIXB

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Validation Data Gallery

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  WB analysis of PC-12 using 14092-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14092-1-AP

  WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negatove control and ARHGEF7 overexpressed Transfected HEK-293 cells.

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  WB analysis of mouse skeletal muscle using 14092-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14092-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14092-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14092-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14092-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14092-1-AP

  IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 14092-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14092-1-AP targets ARHGEF7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGEF7 fusion protein Ag5223
• Full Name: Rho guanine nucleotide exchange factor (GEF) 7
• Calculated Molecular Weight: 803 aa, 90 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC060776
• Gene Symbol: ARHGEF7
• Gene ID (NCBI): 8874
• RRID: AB_2227567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.; Authors - Ruth Chia
human	WB,IHC	Am J Transl Res; Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.; Authors - Tao Ma