PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 14092-1-AP
WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.
WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.
WB analysis of NIH/3T3 using 14092-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14092-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14092-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14092-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14092-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14092-1-AP
IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14092-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, human rectal cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 14092-1-AP
WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.
WB analysis of NIH/3T3 using 14092-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14092-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14092-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14092-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14092-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 14092-1-AP
IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.
IF/ICC Figures
IF Staining of HeLa using 14092-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ARHGEF7 Polyclonal antibody
Catalog Number
14092-1-AP
Citations
3
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
