ARHGEF7 Polyclonal antibody

ARHGEF7 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 14092-1-AP

Synonyms

ARHGEF7, BETA PIX, COOL 1, COOL1, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PAK3BP, PIXB



Tested Applications

Positive WB detected inPC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14092-1-AP targets ARHGEF7 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF7 fusion protein Ag5223
Full Name Rho guanine nucleotide exchange factor (GEF) 7
Calculated molecular weight 803 aa, 90 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC060776
Gene symbol ARHGEF7
Gene ID (NCBI) 8874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IF protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IP protocol for ARHGEF7 antibody 14092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.

Authors - Ruth Chia