ARHGEF9-Specific Polyclonal antibody

ARHGEF9-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20042-1-AP

Synonyms

ARHGEF9, ARHGEF9-Specific, COLLYBISTIN, HPEM 2, KIAA0424, PEM 2, PEM 2 homolog, PEM2

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Validation Data Gallery

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  WB analysis using 20042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20042-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20042-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, human brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20042-1-AP targets ARHGEF9-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Cdc42 guanine nucleotide exchange factor (GEF) 9
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_015185
• Gene Symbol: ARHGEF9
• Gene ID (NCBI): 23229
• RRID: AB_10694145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF9, also named as KIAA0424, acts as guanine nucleotide exchange factor (GEF) for CDC42. ARHGEF9 promotes formation of GPHN clusters. This antibody is specific to ARHGEF9.

Publications

Species	Application	Title
mouse	WB	Synapse; Triptolide injection reduces Alzheimer's disease-like pathology in mice; Authors - Rui Mao