ARHGEF9-Specific Polyclonal antibody

• 

  WB analysis of HeLa using 20042-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20042-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ARHGEF9-Specific Polyclonal Antibody for WB, ELISA

Cat No. 20042-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ARHGEF9, ARHGEF9-Specific, COLLYBISTIN, HPEM 2, KIAA0424, PEM 2, PEM 2 homolog, PEM2

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, human brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20042-1-AP targets ARHGEF9-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Cdc42 guanine nucleotide exchange factor (GEF) 9
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_015185
• Gene Symbol: ARHGEF9
• Gene ID (NCBI): 23229
• RRID: AB_10694145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43307
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF9, also named as KIAA0424, acts as guanine nucleotide exchange factor (GEF) for CDC42. ARHGEF9 promotes formation of GPHN clusters. This antibody is specific to ARHGEF9.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Synapse; Triptolide injection reduces Alzheimer's disease-like pathology in mice; Authors - Rui Mao