ARID1B Recombinant monoclonal antibody, PBS Only

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• ARID1B Recombinant monoclonal antibody, PBS Only (82979-6-PBS)

ARID1B Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82979-6-PBS Copied!

Clone No. 230222A11

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230222A11, BAF250b, BRIGHT, DAN15, ELD/OSA1

• 

  IHC staining of human placenta using 82979-6-RR (same clone as 82979-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 82979-6-RR (same clone as 82979-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse adrenal gland using 82979-6-RR (same clone as 82979-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 82979-6-RR (same clone as 82979-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat cerebellum using 82979-6-RR (same clone as 82979-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 82979-6-RR (same clone as 82979-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1B antibody (82979-6-RR, Clone: 230222A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of SH-SY5Y using 82979-6-RR (same clone as 82979-6-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug ARID1B Recombinant antibody (82979-6-RR, Clone:230222A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 82979-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82979-6-RR against Human ARID1B were performed. The affinity constant is 1.14 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

82979-6-PBS targets ARID1B in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30643

  Product name: Recombinant human ARID1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1370-1508 aa of NM_017519

  Sequence: PAKRHEGDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQTHGIPPQMMGGPLQSSSSEGPQQNMWAARNDMPYPYQNRQGPGGPTQAPPYPGMNRTDDMMVPDQRINHESQWPSHVSQRQP

• Full Name: AT rich interactive domain 1B (SWI1-like)
• Calculated Molecular Weight: 236 kDa
• GenBank Accession Number: NM_017519
• Gene Symbol: ARID1B
• Gene ID (NCBI): 57492
• RRID: AB_3670728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NFD5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.