Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14949-1-AP targets ARIH1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6760 Product name: Recombinant human ARIH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-507 aa of BC051877 Sequence: MQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSG Predict reactive species |
| Full Name | ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC051877 |
| Gene Symbol | ARIH1 |
| Gene ID (NCBI) | 25820 |
| RRID | AB_3085447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4X5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARIH1 (Ariadne-1 homolog) is a member of the Ariadne family of E3 ubiquitin ligases with a cognate E2 enzyme UBCH79,10,11. ARIH1 plays a role in protein translation regulation in response to DNA damage and to ubiquitinate EIF4E2 (PMID: 15236971, PMID: 1532592, PMID: 24076655, PMID: 27565346, PMID: 23707686). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation (PMID: 33879767).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARIH1 antibody 14949-1-AP | Download protocol |
| WB protocol for ARIH1 antibody 14949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARIH1 Polyclonal antibody (Cat# 14949-1-AP) has 6 citations published in Cell Reports, EMBO Reports, Cell Oncology, Cancers, FASEB Journal, and bioRxiv. Research fields span innate immunity and ISGylation, ER stress and proteostasis, HER2-positive lung cancer, breast cancer microtubule biology, preeclampsia-related mitophagy, and viral immune signaling. Applications include WB, CoIP, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cell Rep HERC5-catalyzed ISGylation potentiates cGAS-mediated innate immunity
| ||
EMBO Rep Sec61β maintains cytoplasmic proteostasis via ARIH1-mediated translational repression upon ER stress.
| ||
Cell Oncol (Dordr) Pyrotinib inhibits the tumorigenicity of HER2-positive non-small cell lung cancer by inducing ARIH1/ubiquitin/lysosome-dependent degradation of HER2.
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Cancers (Basel) ARIH1 Inhibition Promotes Microtubule Stability and Sensitizes Breast Cancer Cells to Microtubule-Stabilizing Agents
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FASEB J ARIH1 Promotes Preeclampsia by Inducing MFN2-Dependent Hypoxia-Triggered Mitophagy and Endoplasmic Reticulum Stress in Trophoblasts. | ||
bioRxiv MDA5 ISGylation is crucial for immune signaling to control viral replication and pathogenesis
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Reviews
What customers say
The single review rates this antibody 4 out of 5 for Western Blot on mouse brain lysate at a 1:3000 dilution. The reviewer confirmed it detects endogenous ARIH1 at the expected ~60 kDa molecular weight, though noted some weak non-specific bands below 37 kDa. Overall, the antibody performed well for its intended target with minor background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nou (Verified Customer) (01-02-2026) | This Ab can detect endo ARIH1 with the right MW of around 60 kD, although there are some weak non-specific bands below 37 kD.
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