ARIH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARIH2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15006-1-AP

Synonyms

EC:2.3.2.31, E3 ubiquitin-protein ligase ARIH2, ARIH 2, ARI-2, ARI2

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Validation Data Gallery

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  WB analysis using 15006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15006-1-AP (ARIH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15006-1-AP

  IP result of anti-ARIH2 (IP:15006-1-AP, 4ug; Detection:15006-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HEK-293 using 15006-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15006-1-AP (ARIH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

15006-1-AP targets ARIH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARIH2 fusion protein Ag6507
• Full Name: ariadne homolog 2 (Drosophila)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC000422
• Gene Symbol: ARIH2
• Gene ID (NCBI): 10425
• RRID: AB_2290024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.

Publications

Species	Application	Title
mouse	WB,IP	Nat Immunol; ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system.; Authors - Lin Amy E AE
mouse	WB	Oncogene; The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.; Authors - Hao Wang
human	WB,IHC,IP	Cell Death Dis; ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.; Authors - Shengjun Geng ; KD Validated