Tested Applications
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, mouse liver tissue, HepG2 cells, Neuro-2a cells, C6 cells |
| Positive IP detected in | Neuro-2a cells |
| Positive IHC detected in | human stomach cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16012-1-AP targets ARL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8770 Product name: Recombinant human ARL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC007000 Sequence: MGGFFSSIFSSLFGTREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVETVTYKNLKFQVWDLGGQTSIRPYWRCYYSNTDAVIYVVDSCDRDRIGISKSELVAMLEEEELRKAILVVFANKQDMEQAMTSSEMANSLGLPALKDRKWQIFKTSATKGTGLDEAMEWLVETLKSRQ Predict reactive species |
| Full Name | ADP-ribosylation factor-like 1 |
| Calculated Molecular Weight | 181 aa, 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC007000 |
| Gene Symbol | ARL1 |
| Gene ID (NCBI) | 400 |
| RRID | AB_2243131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40616 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARL1 antibody 16012-1-AP | Download protocol |
| IHC protocol for ARL1 antibody 16012-1-AP | Download protocol |
| IP protocol for ARL1 antibody 16012-1-AP | Download protocol |
| WB protocol for ARL1 antibody 16012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARL1 Polyclonal antibody (Cat# 16012-1-AP) has 13 citations spanning journals including Nature, Cell, Nature Cell Biology, Nature Protocols, Nature Communications, Cell Discovery, Cell Chemical Biology, Cell Reports, Molecular & Cellular Proteomics, Journal of Cell Biology, Frontiers in Physiology, and Biological Cell. Research fields encompass protein N-myristoylation, vesicle trafficking, trans-Golgi network organization, ferroptosis, cilia transport, STING signaling, and actin-dependent organelle dynamics.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells | ||
Nat Protoc Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation. | ||
Nat Commun Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins. | ||
Cell Discov Trans-Golgi network tethering factors regulate TBK1 trafficking and promote the STING-IFN-I pathway |
