Tested Applications
| Positive WB detected in | mouse thymus tissue |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11934-1-AP targets ARL15 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2530 Product name: Recombinant human ARL15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC026093 Sequence: MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNVVSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAARNELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDMDALKDSFSQLINLLEEKDHEAVRM Predict reactive species |
| Full Name | ADP-ribosylation factor-like 15 |
| Calculated Molecular Weight | 204 aa, 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC026093 |
| Gene Symbol | ARL15 |
| Gene ID (NCBI) | 54622 |
| RRID | AB_2227438 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARL15(ADP-ribosylation factor-like protein 15) is also named as ARFRP2(ADP-ribosylation factor-related protein 2) and belongs to the small GTPase superfamily and Arf familiy.ADP-ribosylation factor-related protein (ARP) is a membrane-associated GTPase with remote similarity to the family of ADP-ribosylation factors (ARF)(PMID:10092663). Arl15 is present in chordates but not lower eukaryotes. Arl15 lacks a site for Nmyristoylation, but the N-terminal region contains several well-conserved cysteines, suggesting that it may bepalmitoylated(PMID:17506703).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARL15 antibody 11934-1-AP | Download protocol |
| IP protocol for ARL15 antibody 11934-1-AP | Download protocol |
| WB protocol for ARL15 antibody 11934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARL15 antibody (Cat# 11934-1-AP) has 6 total citations published in PLoS Biology, Life Sciences, PLoS Genetics, Traffic, Biochemical and Biophysical Research Communications, and Science Advances. Research fields span insulin signaling and oxidative stress in diabetes, adiponectin regulation via GWAS, Golgi cargo transport and cell adhesion, AKT phosphorylation in the IR/IRS1/AKT pathway, TRPM7 channel-mediated divalent cation entry, and extrasynaptic neuronal cilia signaling. Applications include WB, IF, IHC, and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
PLoS Biol CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells.
| ||
PLoS Genet A genome-wide association study reveals variants in ARL15 that influence adiponectin levels. | ||
Biochem Biophys Res Commun ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity. | ||
Sci Adv In situ proteomics unveils specialized domains for extrasynaptic signaling on neuronal cilia. |
