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  • KD/KO Validated

ARL15 Polyclonal antibody

ARL15 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 11934-1-AP

Synonyms

ARF related protein 2, ARFRP2, ARL15



Tested Applications

Positive WB detected inmouse thymus tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11934-1-AP targets ARL15 in WB, IP, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL15 fusion protein Ag2530
Full Name ADP-ribosylation factor-like 15
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC026093
Gene Symbol ARL15
Gene ID (NCBI) 54622
RRIDAB_2227438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL15(ADP-ribosylation factor-like protein 15) is also named as ARFRP2(ADP-ribosylation factor-related protein 2) and belongs to the small GTPase superfamily and Arf familiy.ADP-ribosylation factor-related protein (ARP) is a membrane-associated GTPase with remote similarity to the family of ADP-ribosylation factors (ARF)(PMID:10092663). Arl15 is present in chordates but not lower eukaryotes. Arl15 lacks a site for Nmyristoylation, but the N-terminal region contains several well-conserved cysteines, suggesting that it may bepalmitoylated(PMID:17506703).

Protocols

Product Specific Protocols
WB protocol for ARL15 antibody 11934-1-APDownload protocol
IHC protocol for ARL15 antibody 11934-1-APDownload protocol
IP protocol for ARL15 antibody 11934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

A genome-wide association study reveals variants in ARL15 that influence adiponectin levels.

Authors - Richards J Brent JB
mouseIP

PLoS Biol

CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells.

Authors - Zhiyong Bai
  • KD Validated
humanIHC

Biochem Biophys Res Commun

ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity.

Authors - Jie Zhao
humanWB

Life Sci

PMID: 30682341

Authors - Jiayi Shen
  • KD Validated