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  • KD/KO Validated

ARL2BP Polyclonal antibody

ARL2BP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10090-2-AP

Synonyms

ARF like 2 binding protein, ARL2BP, BART, BART1, Binder of ARF2 protein 1



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10090-2-AP targets ARL2BP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL2BP fusion protein Ag0138
Full Name ADP-ribosylation factor-like 2 binding protein
Calculated molecular weight 19 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC003087
Gene symbol ARL2BP
Gene ID (NCBI) 23568
RRIDAB_2058518
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.

Protocols

Product Specific Protocols
WB protocol for ARL2BP antibody 10090-2-APDownload protocol
IHC protocol for ARL2BP antibody 10090-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Am J Hum Genet

Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.

Authors - Davidson Alice E AE
  • KD Validated
humanWB

Cancer Res

Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.

Authors - Keisuke Taniuchi
humanWB,IF

Neoplasia

BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.

Authors - Taniuchi Keisuke K
  • KD Validated
humanWB,IF

PLoS One

BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.

Authors - Taniuchi Keisuke K
  • KD Validated
humanWB,IF

Invest Ophthalmol Vis Sci

Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.

Authors - Mori Naoki N
humanWB,IF,IP

Int J Oncol

BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.

Authors - Taniuchi Keisuke K