• Featured Product
  • KD/KO Validated

ARL2BP Polyclonal antibody

ARL2BP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10090-2-AP

Synonyms

ARF like 2 binding protein, ARL2BP, BART, BART1, Binder of ARF2 protein 1



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10090-2-AP targets ARL2BP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL2BP fusion protein Ag0138
Full Name ADP-ribosylation factor-like 2 binding protein
Calculated molecular weight 19 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC003087
Gene symbol ARL2BP
Gene ID (NCBI) 23568
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.

Protocols

Product Specific Protocols
WB protocol for ARL2BP antibody 10090-2-APDownload protocol
IHC protocol for ARL2BP antibody 10090-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Int J Oncol

BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.

Authors - Taniuchi Keisuke K
humanWB,IF

Invest Ophthalmol Vis Sci

Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.

Authors - Mori Naoki N
humanWB,IF

PLoS One

BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.

Authors - Taniuchi Keisuke K
  • KD Validated
humanWB,IF

Neoplasia

BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.

Authors - Taniuchi Keisuke K
  • KD Validated
humanWB

Cancer Res

Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.

Authors - Keisuke Taniuchi
mouseWB,IF

Am J Hum Genet

Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.

Authors - Davidson Alice E AE
  • KD Validated