|Positive WB detected in
|Positive IHC detected in
|human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:200-1:1000
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10090-2-AP targets ARL2BP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ARL2BP fusion protein Ag0138
|ADP-ribosylation factor-like 2 binding protein
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.
Am J Hum Genet
Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.
Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.
BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.
BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
Invest Ophthalmol Vis Sci
Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.
Int J Oncol
BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.