|Positive WB detected in||A549 cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10090-2-AP targets ARL2BP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARL2BP fusion protein Ag0138|
|Full Name||ADP-ribosylation factor-like 2 binding protein|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC003087|
|Gene ID (NCBI)||23568|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.
Int J Oncol
BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.
Invest Ophthalmol Vis Sci
Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.
BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.
Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.
Am J Hum Genet
Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.