ARL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10961-1-AP

Synonyms

ADP ribosylation factor like 3, ARFL3, ARL3

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Validation Data Gallery

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  WB analysis using 10961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10961-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10961-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10961-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10961-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10961-1-AP (ARL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10961-1-AP (ARL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of IMCD3 cells using 10961-1-AP

  IF result of 10961-1-AP (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus).

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  IF Staining of hTERT-RPE1 using 10961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using 10961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, PC-3 cells, human brain tissue, mouse testis tissue, rat brain tissue, HeLa cells, rat testis tissue
• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells, IMCD3 cells, hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

10961-1-AP targets ARL3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL3 fusion protein Ag1406
• Full Name: ADP-ribosylation factor-like 3
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20 kDa, 25 kDa
• GenBank Accession Number: BC009841
• Gene Symbol: ARL3
• Gene ID (NCBI): 403
• RRID: AB_2274220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB,IF	Nat Commun; Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.; Authors - Antonios D Konitsiotis
mouse	WB,IF, IP	Nat Commun; Arl3 and LC8 regulate dissociation of dynactin from dynein.; Authors - Mingyue Jin ; KD Validated
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim ; KD Validated
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC
human	IF	Dev Cell; The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.; Authors - Louise A Stephen