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  • KD/KO Validated

ARL8A Polyclonal antibody

ARL8A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 17060-1-AP

Synonyms

ARL10B, ARL8A, GIE2



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, HeLa cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17060-1-AP targets ARL8A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL8A fusion protein Ag10788
Full Name ADP-ribosylation factor-like 8A
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC015408
Gene Symbol ARL8A
Gene ID (NCBI) 127829
RRIDAB_2058998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A has a calculated molecular weight of 21 kDa.

Protocols

Product Specific Protocols
WB protocol for ARL8A antibody 17060-1-APDownload protocol
IP protocol for ARL8A antibody 17060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.

Authors - Tal Keren-Kaplan
humanWB

Curr Biol

ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1.

Authors - Tal Keren-Kaplan
human,ratIF

J Cell Biol

Specific KIF1A-adaptor interactions control selective cargo recognition.

Authors - Jessica J A Hummel
  • KD Validated
humanWB

J Cell Sci

The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.

Authors - Ziyan Fang
  • KD Validated