ARMC2 Polyclonal antibody

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• ARMC2 Polyclonal antibody (24011-1-AP)

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  WB analysis using 24011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24011-1-AP (ARMC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 24011-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using ARMC2 antibody (24011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 24011-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ARMC2 antibody (24011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ARMC2 Polyclonal Antibody for WB, IF-Fro, ELISA

Cat No. 24011-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-Fro, ELISA

Synonyms

armadillo repeat containing 2, ARMC 2, RP11 787I22.1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, rat testis tissue
• Positive IF-Fro detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24011-1-AP targets ARMC2 in WB, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21162

  Product name: Recombinant human ARMC2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 510-860 aa of BC030603

  Sequence: CYALFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSIQTLLSLFQTFHQLDLHSQKPVGQRGEQHRAQRPPSEAEDVLIKLTRVLANIAIHPGVGPVLAANPGIVGLLLTTLEYKSLDDCEELVINATATINNLSYYQVKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGGIKKLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEELALDGSFDPDLKNYHKLHWETEFKPVAQQLLNRIQRHHTFLEPLPIPSF

• Full Name: armadillo repeat containing 2
• Calculated Molecular Weight: 867 aa, 97 kDa
• Observed Molecular Weight: 78 kDa,97 kDa
• GenBank Accession Number: BC030603
• Gene Symbol: ARMC2
• Gene ID (NCBI): 84071
• RRID: AB_3669428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8NEN0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ARMC2 protein (Armadillo Repeat Containing 2) is an evolutionarily conserved axonemal-related protein that plays a crucial role in the structure and function of cilia and flagella. It interacts with the kinesin-2 complex through its unique Armadillo repeat domain and acts as an adapter for the flagellar transport (IFT) process, responsible for transporting specific cargoes (such as the precursor proteins necessary for the construction of flagellar axons) from the cell body to the flagellar tip, which is essential for the assembly, maintenance, and signal transduction functions of the flagella. Its dysfunction is directly related to abnormal sperm flagellar morphology and movement disorders.