Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12653-1-AP targets ARMC8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3374 Product name: Recombinant human ARMC8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC013424 Sequence: MEVTASSRHYVDRLFDPDPQKVLQGVIDMKNAVIGNNKQKANLIVLGAVPRLLYLLQQETSSTELKTECAVVLGSLAMGTENNVKSLLDCHIIPALLQGLLSPDLKFIEACLRCLRTIFTSPVTPEELLYTDATVIPHLMALLSRSRYTQEYICQIFSHCCKGPDHQTILFNHGAVQNIAHLLTSLSYKVRMQALKCFSVLAFENPQVSMTLVNVLVDGELLPQIFVKMLQRDKPIEMQLTSAKCLTYMCRAGAIRTDDNCIVLKTLPCLVRMCSKERLLEERVEGAETLAYLIEPDVELQRIASITDHLIAMLADYFKYPSSVSAITDIKRLDHDLKHAHELRQAAFKL Predict reactive species |
| Full Name | armadillo repeat containing 8 |
| Calculated Molecular Weight | 673 aa, 75.5 kDa |
| Observed Molecular Weight | 75.5 kDa |
| GenBank Accession Number | BC013424 |
| Gene Symbol | ARMC8 |
| Gene ID (NCBI) | 25852 |
| RRID | AB_2059154 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUR7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Armadillo repeat-containing protein 8 (Armc8) is a key factor to regulate cell membrane adhesion complex through promoting α-catenin degradation. Armc8 expression was elevated during atypia-to-carcinoma progression and may be associated with cancer development of breast carcinoma (PMID: 25119601).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARMC8 antibody 12653-1-AP | Download protocol |
| WB protocol for ARMC8 antibody 12653-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARMC8 Polyclonal antibody (Cat# 12653-1-AP) has 5 citations across journals including Molecular Cell, Cell Reports, Scientific Reports, Journal of Dermatological Science, and Dose-Response. Research fields span mTORC1/CTLH E3 ligase-mediated protein degradation, pyrimidine salvage pathway regulation, Ran Binding Protein 9–Nucleolin interactions, Wnt/β-catenin signaling and EMT in cutaneous squamous cell carcinoma, and UVB-induced cellular damage. All cited studies used human samples with WB, IHC, or IP applications.
| Species | Application | Title |
|---|---|---|
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway | ||
Cell Rep mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase | ||
Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. | ||
J Dermatol Sci Downregulation of ARMC8 promotes tumorigenesis through activating Wnt/β-catenin pathway and EMT in cutaneous squamous cell carcinomas. | ||
Dose Response MiR-664 Protects Against UVB Radiation-Induced HaCaT Cell Damage via Downregulating ARMC8.
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Reviews
What customers say
Both reviewers used this antibody for Western Blot on HEK293T cells at a 1:2000 dilution. One rated it 5 stars, noting it works quite well but may show a few off-target bands if sample loading is excessive. The other gave 4 stars, highlighting that it detects both ARMC8a and ARMC8b isoforms and produces clear signal intensity, making it easy to identify the correct bands immediately. Overall, users report strong specific signal with minor caveats around sample loading.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (12-31-2021) | This antibody works quite well. There are a few off target bands though, if the samples are loaded too much.
 |
H (Verified Customer) (09-27-2020) | This antibody detects both ARMC8a and b. The signal was good enough to tell which are the right bands immediately.
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