ARMET Polyclonal antibody

• 

  WB analysis of mouse pancreas using 10869-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10869-1-AP (ARMET Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 10869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10869-1-AP (ARMET antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10869-1-AP (ARMET antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ARMET Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10869-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

Arginine rich protein, ARMET, ARP, MANF, Protein ARMET

---

Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10869-1-AP targets ARMET in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1115

  Product name: Recombinant human ARMET protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-182 aa of BC007282

  Sequence: MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

• Full Name: arginine-rich, mutated in early stage tumors
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC007282
• Gene Symbol: ARMET
• Gene ID (NCBI): 7873
• RRID: AB_2059300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55145
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Imbalanced unfolded protein response signaling contributes to 1-deoxysphingolipid retinal toxicity; Authors - Jessica D Rosarda
		J Am Soc Nephrol; Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress-Related Kidney Diseases.; Authors - Yeawon Kim
human	IHC	J Hepatocell Carcinoma; Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology.; Authors - Dandan Zhao
human	WB	Eur J Oral Sci; Effect of mesencephalic astrocyte-derived neurotrophic factor on the inflammatory response in human gingival fibroblasts cells; Authors - Lei Gong ; KD Validated
mouse	WB	Int Immunopharmacol; Mesencephalic astrocyte-derived neurotrophic factor (MANF) alleviates cerebral ischemia/reperfusion injury in mice by regulating microglia polarization via A20/NF-κB pathway; Authors - Xueyan Zhao
human	WB	Front Oncol; DLC1 inhibits colon adenocarcinoma cell migration by promoting secretion of the neurotrophic factor MANF; Authors - Yi-Min Chu