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ARNT,HIF1B Polyclonal antibody

ARNT,HIF1B Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14105-1-AP

Synonyms

ARNT, ARNT protein, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14105-1-AP targets ARNT,HIF1B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARNT,HIF1B fusion protein Ag5238
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC060838
Gene symbol ARNT
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
WB protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
IHC protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
IF protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
IP protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hepatology

PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.

Authors - Liu Yanfeng Y
humanWB,IF

BMC Med

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan
humanWB

Free Radic Biol Med

Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.

Authors - Kyuhwa Seo
mouseWB

Front Microbiol

Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.

Authors - Zhixiao Sun
mouseWB

J Biomed Nanotechnol

Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.

Authors - Zhongqi Chen
human,mouseIHC

Mol Hum Reprod

Induction of aryl hydrocarbon receptor in granulosa cells by endoplasmic reticulum stress contributes to pathology of polycystic ovary syndrome.

Authors - Chisato Kunitomi