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ARNT,HIF1B Polyclonal antibody

ARNT,HIF1B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 14105-1-AP


ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14105-1-AP targets ARNT,HIF1B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARNT,HIF1B fusion protein Ag5238
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia


Product Specific Protocols
WB protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
IHC protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
IF protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
IP protocol for ARNT,HIF1B antibody 14105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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