ARNTL Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86113-1-RR (same clone as 86113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86113-1-RR (ARNTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

  WB result of ARNTL antibody (86113-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNTL transfected HeLa cells. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86113-1-RR (same clone as 86113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 86113-1-RR (same clone as 86113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARNTL antibody (86113-1-RR, Clone: 250536E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ARNTL Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86113-1-PBS

Clone No.250536E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BMAL1, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

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Product Information

86113-1-PBS targets ARNTL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5586

  Product name: Recombinant human ARNTL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-303 aa of BC041129

  Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP

• Full Name: aryl hydrocarbon receptor nuclear translocator-like
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC041129
• Gene Symbol: ARNTL
• Gene ID (NCBI): 406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00327
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).