ARPC4 Polyclonal antibody

ARPC4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10930-1-AP

Synonyms

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc



Tested Applications

Positive WB detected inmouse brain tissue, mouse small intestine tissue, rat lymph tissue, 4T1 cells, human placenta tissue, human brain tissue, mouse cerebellum tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10930-1-AP targets ARPC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPC4 fusion protein Ag1366
Full Name actin related protein 2/3 complex, subunit 4, 20kDa
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 19-25 kDa
GenBank Accession NumberBC012596
Gene Symbol ARPC4
Gene ID (NCBI) 10093
RRIDAB_2059951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARPC4 antibody 10930-1-APDownload protocol
IHC protocol for ARPC4 antibody 10930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells

Authors - Sumana Sharma
humanWB

Biochim Biophys Acta Proteins Proteom

Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis.

Authors - Guoxuan Qin