ARPC4 Polyclonal antibody

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10930-1-AP

Publications (9)

Review Product

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse brain using 10930-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 10930-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 10930-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse small intestine using 10930-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse cerebellum using 10930-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat lymph using 10930-1-AP
rat lymph tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 10930-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 10930-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of 4T1 using 10930-1-AP
4T1 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human urothelial carcinoma using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for ARPC4 in ?

Overview

Tested Applications
Product Information
Publications (9)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse small intestine tissue, rat lymph tissue, 4T1 cells, human placenta tissue, human brain tissue, mouse cerebellum tissue, MCF-7 cells
Positive IHC detected in	human cervical cancer tissue, human testis tissue, human lung cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10930-1-AP targets ARPC4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1366 Product name: Recombinant human ARPC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC012596 Sequence: TATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFTMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF Predict reactive species
Full Name	actin related protein 2/3 complex, subunit 4, 20kDa
Calculated Molecular Weight	20 kDa
Observed Molecular Weight	19-25 kDa
GenBank Accession Number	BC012596
Gene Symbol	ARPC4
Gene ID (NCBI)	10093
RRID	AB_2059951
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P59998
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).

Protocols

Product Specific Protocols
IHC protocol for ARPC4 antibody 10930-1-AP	Download protocol
WB protocol for ARPC4 antibody 10930-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ARPC4 polyclonal antibody (Cat# 10930-1-AP) has 9 citations across journals including Drug Resistance Updates, Advanced Science, Science Advances, Nature Structural & Molecular Biology, Biomolecules, Life Science Alliance, Biochimica et Biophysica Acta, and International Journal of Molecular Sciences. Research fields span drug resistance in gliomas, oocyte development, alternative splicing in CML, cancer metastasis, mastitis inflammation, T-cell signaling, hepatocellular carcinoma, and cytoskeletal/inflammasome regulation.

Species	Application	Title
human	WB	Drug Resist Updat Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane Authors - Xin Zhang View Article
mouse	WB,IF	Adv Sci (Weinh) Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice. Authors - Rui Xu View Article
human	WB	Sci Adv Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib. Authors - Paulina Podszywałow-Bartnicka View Article
human	WB,IP	Nat Struct Mol Biol Akt-phosphorylated UFL1 UFMylates ArpC4 to promote metastasis Authors - Kailiang Zhao View Article
mouse,bovine	WB,IF	Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model Authors - Bo Fang View Article
mouse,bovine	WB,IF	Biomolecules Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis Authors - Tingji Yang View Article