ARPC4 Polyclonal antibody

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  WB analysis of mouse brain using 10930-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10930-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10930-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 10930-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10930-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10930-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of 4T1 using 10930-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of rat lymph using 10930-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10930-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10930-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse small intestine tissue, rat lymph tissue, 4T1 cells, human placenta tissue, human brain tissue, mouse cerebellum tissue, MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue, human testis tissue, human lung cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below

Product Information

10930-1-AP targets ARPC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1366

  Product name: Recombinant human ARPC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-167 aa of BC012596

  Sequence: TATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFTMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF

• Full Name: actin related protein 2/3 complex, subunit 4, 20kDa
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 19-25 kDa
• GenBank Accession Number: BC012596
• Gene Symbol: ARPC4
• Gene ID (NCBI): 10093
• RRID: AB_2059951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P59998
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Life Sci Alliance; A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells; Authors - Sumana Sharma
human	WB	Biochim Biophys Acta Proteins Proteom; Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis.; Authors - Guoxuan Qin
human	WB	Drug Resist Updat; Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane; Authors - Xin Zhang
mouse,bovine	WB,IF	Biomolecules; Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model; Authors - Bo Fang
mouse,bovine	WB,IF	Biomolecules; Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis; Authors - Tingji Yang