ARPP-19 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARPP-19 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 11678-1-AP

Synonyms

ARPP19, cAMP-regulated phosphoprotein 19, ARPP16, ARPP 19, ARPP 16

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Validation Data Gallery

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  WB analysis of Jurkat using 11678-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11678-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11678-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11678-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 11678-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11678-1-AP

  IP result of anti-ARPP-19 (IP:11678-1-AP, 3ug; Detection:11678-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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  IHC staining of human gliomas using 11678-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11678-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 11678-1-AP

  Immunofluorescent analysis of A375 cells using 11678-1-AP (ARPP-19 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, A375 cells, A549 cells, mouse brain tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

11678-1-AP targets ARPP-19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARPP-19 fusion protein Ag2279
• Full Name: cyclic AMP phosphoprotein, 19 kD
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC003418
• Gene Symbol: ARPP-19
• Gene ID (NCBI): 10776
• RRID: AB_2060078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARPP-19, a 19-kDa cAMP-regulated phosphoprotein, plays a role in regulating mitosis. Initiation and maintenance of mitosis require the activation of protein kinase cyclin B-Cdc2 and the inhibition of protein phosphatase 2A (PP2A). When phosphorylated by protein kinase Greatwall (Gwl), ARPP-19 associate with and inhibit PP2A, thus promoting mitotic entry. ARPP-19 may be an important link between nerve growth factor (NGF) signaling and post-transcriptional control of neuronal gene expression such as GAP-43.

Publications

Species	Application	Title
human	WB	Front Oncol; Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.; Authors - Xigang Xia
human	WB	J Proteome Res; Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression.; Authors - Luqi Jin
human	WB,IHC	Onco Targets Ther; PMID: 32753897; Authors - Xiang Gao ; KD Validated
human	WB	Int J Mol Sci; Increased ARPP-19 expression is associated with hepatocellular carcinoma.; Authors - Haiyan Song
human	WB	Cancers (Basel); Arpp19 Promotes Myc and Cip2a Expression and Associates with Patient Relapse in Acute Myeloid Leukemia.; Authors - Eleonora Mäkelä ; KD Validated
human	WB,IP	Nature; Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19; Authors - Sathish K R Padi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

ARPP19 antibody giving a very clear sharp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer cells