ARPP-21 Polyclonal antibody

ARPP-21 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11829-1-AP

Synonyms

ARPP 21, ARPP21, ARPP-21, RCS, TARPP

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Validation Data Gallery

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  WB analysis of mouse brain using 11829-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11829-1-AP (ARPP-21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11829-1-AP (ARPP-21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11829-1-AP (ARPP-21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 11829-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11829-1-AP (ARPP-21 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cerebellum using 11829-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11829-1-AP (ARPP-21 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11829-1-AP targets ARPP-21 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARPP-21 fusion protein Ag2399
• Full Name: cyclic AMP-regulated phosphoprotein, 21 kD
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC017805
• Gene Symbol: ARPP-21
• Gene ID (NCBI): 10777
• RRID: AB_2877798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.; Authors - Ye-Ya Tan
mouse	WB	Nat Commun; The thymocyte-specific RNA-binding protein Arpp21 provides TCR repertoire diversity by binding to the 3'-UTR and promoting Rag1 mRNA expression; Authors - Meng Xu