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  • KD/KO Validated

Beta Arrestin 2 Polyclonal antibody

Beta Arrestin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10171-1-AP

Synonyms

ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2, β-Arrestin 2



Tested Applications

Positive WB detected inmouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue
Positive IHC detected inhuman tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse Retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Arrestin 2 fusion protein Ag0227
Full Name arrestin, beta 2
Calculated molecular weight 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC007427
Gene symbol ARRB2
Gene ID (NCBI) 409
RRIDAB_10644158
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Protocols

Product Specific Protocols
WB protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IHC protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IF protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.

Authors - Yuqing Wu
  • KO Validated
ratWB

Autophagy

Autophagy and post-ischemic conditioning in retinal ischemia.

Authors - Biji Mathew
human,mouseWB,IF,CoIP

J Pharm Anal

Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis

Authors - Jia-Jia Zhang
  • KD Validated
mouseIF

J Cell Biol

BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.

Authors - Fan Ye
mouseIF

Acta Pharmacol Sin

Activation of GPR120 in podocytes ameliorates kidney fibrosis and inflammation in diabetic nephropathy.

Authors - Tian-Tian Wei
humanWB

Development

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Authors - Verena Rauschenberger