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Beta Arrestin 2 Polyclonal antibody
Beta Arrestin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10171-1-AP
Validation Data Gallery
|Positive WB detected in||RAW 264.7 cells, rat brain tissue, PC-12 cells, RAW264.7|
|Positive IP detected in||RAW 264.7 cells|
|Positive IHC detected in||human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse Retina tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beta Arrestin 2 fusion protein Ag0227|
|Full Name||arrestin, beta 2|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC007427|
|Gene ID (NCBI)||409|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
Acta Pharmacol Sin
Activation of GPR120 in podocytes ameliorates kidney fibrosis and inflammation in diabetic nephropathy.
Bioactive peptide apelin rescues acute kidney injury by protecting the function of renal tubular mitochondria.
β-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca(2+) Pathway in Xenopus Gastrulation.
J Biol Chem
An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.
Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the α7nAch receptor in SH-SY5Y cells.
Lactate enhances Arc/arg3.1 expression through hydroxycarboxylic acid receptor 1-β-arrestin2 pathway in astrocytes.