Beta Arrestin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Beta Arrestin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10171-1-AP

Synonyms

ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2

---

Validation Data Gallery

• 

  WB analysis using 10171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 10171-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10171-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10171-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10171-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsil using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsil using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse Retina tissue using 10171-1-AP

  IF result of anti-ARRB2 (10171-1-AP) in Mouse Retina by Dr. Seongjin Seo. IS (Inner segment) Stain.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue
• Positive IHC detected in: human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse Retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Arrestin 2 fusion protein Ag0227
• Full Name: arrestin, beta 2
• Calculated molecular weight: 46 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC007427
• Gene symbol: ARRB2
• Gene ID (NCBI): 409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.; Authors - Yuqing Wu ; KO Validated
rat	WB	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
mouse	IF	J Cell Biol; BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.; Authors - Fan Ye
human	WB	Development; The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.; Authors - Verena Rauschenberger
mouse	WB,IHC	Front Immunol; Sympathetic Denervation Ameliorates Renal Fibrosis via Inhibition of Cellular Senescence.; Authors - Qian Li
mouse	WB,IHC,IF	Cell Death Dis; β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.; Authors - J Liu ; KD Validated

Related Products

Beta Arrestin 1 Polyclonal antibody

15361-1-AP

MDM2 Polyclonal antibody

19058-1-AP

AGTR1 Polyclonal antibody

25343-1-AP

ADRB2 Polyclonal antibody

13096-1-AP

GRK2 Polyclonal antibody

13990-1-AP

ERK1/2 Polyclonal antibody

16443-1-AP

Arrestin C Polyclonal antibody

11100-2-AP