ARSI Polyclonal antibody

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• ARSI Polyclonal antibody (25112-1-AP)

• 

  WB analysis of A549 using 25112-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25112-1-AP (ARSI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 25112-1-AP

  IP result of anti-ARSI (IP:25112-1-AP, 4ug; Detection:25112-1-AP 1:500) with A549 cells lysate 800 ug.

ARSI Polyclonal Antibody for WB, IP, ELISA

Cat No. 25112-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Arylsulfatase I, ASI, EC:3.1.6.-

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25112-1-AP targets ARSI in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18884

  Product name: Recombinant human ARSI protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 220-569 aa of BC129995

  Sequence: LASHSPQRPLFLYVAFQAVHTPLQSPREYLYRYRTMGNVARRKYAAMVTCMDEAVRNITWALKRYGFYNNSVIIFSSDNGGQTFSGGSNWPLRGRKGTYWEGGVRGLGFVHSPLLKRKQRTSRALMHITDWYPTLVGLAGGTTSAADGLDGYDVWPAISEGRASPRTEILHNIDPLYNHAQHGSLEGGFGIWNTAVQAAIRVGEWKLLTGDPGYGDWIPPQTLATFPGSWWNLERMASVRQAVWLFNISADPYEREDLAGQRPDVVRTLLARLAEYNRTAIPVRYPAENPRAHPDFNGGAWGPWASDEEEEEEEGRARSFSRGRRKKKCKICKLRSFFRKLNTRLMSQRI

• Full Name: arylsulfatase family, member I
• Calculated Molecular Weight: 569 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC129995
• Gene Symbol: ARSI
• Gene ID (NCBI): 340075
• RRID: AB_3742136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5FYB1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.