ART3 Recombinant monoclonal antibody

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• ART3 Recombinant monoclonal antibody (87108-1-RR)

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  WB analysis of mouse testis using 87108-1-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 87108-1-RR (ART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity of 87108-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87108-1-RR against Human ART3 were performed. The affinity constant is 0.205 nM.

ART3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87108-1-RR

Clone No.252232A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ADP ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, EC:2.4.2.31, Ecto ADP ribosyltransferase 3

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Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87108-1-RR targets ART3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8775

  Product name: Recombinant human ART3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-364 aa of BC008397

  Sequence: EVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPVPVPGPKSHPSASLG

• Full Name: ADP-ribosyltransferase 3
• Calculated Molecular Weight: 389 aa, 44 kDa
• Observed Molecular Weight: 38-47 kDa
• GenBank Accession Number: BC008397
• Gene Symbol: ART3
• Gene ID (NCBI): 419
• RRID: AB_3745345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13508
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ART3 is a GPI-anchored mono-ADP-ribosyltransferase enriched on the sperm cell surface, where it participates in spermatid maturation and membrane remodeling. Localized to lipid rafts, ART3 likely modulates cell-cell signaling and extracellular protein modification. Its upregulation in multiple cancers suggests additional roles in migration, invasion, and tumor aggressiveness. Despite incomplete characterization of its enzymatic activity, ART3 remains a key regulator at the interface of reproductive biology, membrane signaling, and pathology.