ART5 Polyclonal antibody

ART5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11354-1-AP

Synonyms

ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 11354-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11354-1-AP (ART5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 11354-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11354-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11354-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11354-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11354-1-AP targets ART5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ART5 fusion protein Ag1786
• Full Name: ADP-ribosyltransferase 5
• Calculated Molecular Weight: 292 aa, 32 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014577
• Gene Symbol: ART5
• Gene ID (NCBI): 116969
• RRID: AB_10734329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ART5 (Ecto-ADP-ribosyltransferase 5) belongs to a class of enzymes that catalyze the transfer of an ADP-ribose group from NAD to arginine residues on target proteins like G proteins. ART5 is a secreted protein. This antibody detects the 43 kDa ART5 protein in mouse liver and testis.