Tested Applications
| Positive WB detected in | mouse small intestine tissue |
| Positive IHC detected in | human pancreas cancer tissue, human appendicitis tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27742-1-AP targets ASAH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26908 Product name: Recombinant human ASAH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-333 aa of BC107105 Sequence: GVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKG Predict reactive species |
| Full Name | N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 |
| Calculated Molecular Weight | 780 aa, 86 kDa |
| Observed Molecular Weight | 100-115 kDa |
| GenBank Accession Number | BC107105 |
| Gene Symbol | ASAH2 |
| Gene ID (NCBI) | 56624 |
| RRID | AB_2918129 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR71 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASAH2 antibody 27742-1-AP | Download protocol |
| WB protocol for ASAH2 antibody 27742-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASAH2 Polyclonal antibody (Cat# 27742-1-AP) has 2 citations. One appears in Life Metabolism (2024), studying ceramide breakdown in skeletal muscle related to high-fat diet-induced obesity in mice. The other is in Cancer Prevention & Research (2024), identifying stool protein biomarkers for early detection of diffuse-type gastric cancer in humans and mice. Both citations use the antibody for Western blot, spanning metabolic disease and oncology research fields.
| Species | Application | Title |
|---|---|---|
Life Metab High temperature ameliorates high-fat diet-induced obesity by promoting ceramide breakdown in skeletal muscle tissue | ||
Cancer Prev Res (Phila) Stool Protein Mass Spectrometry Identifies Biomarkers for Early Detection of Diffuse-type Gastric Cancer |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody for Western Blot at a 1:2000 dilution on mouse small intestine tissue and described it simply as a "good antibody." Overall, the feedback indicates reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (01-20-2023) | Good antibody
 |
