ASAP3 Polyclonal antibody, PBS Only

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• ASAP3 Polyclonal antibody, PBS Only (14099-1-PBS)

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  WB analysis using 14099-1-AP (same clone as 14099-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14099-1-AP (ASAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14099-1-AP (same clone as 14099-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASAP3 antibody (14099-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

ASAP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14099-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Development and differentiation-enhancing factor-like 1, DDEFL1, CENTB6, ASAP 3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

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Product Information

14099-1-PBS targets ASAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5231

  Product name: Recombinant human ASAP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 558-903 aa of BC060786

  Sequence: RDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD

• Full Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
• Calculated Molecular Weight: 903 aa, 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC060786
• Gene Symbol: ASAP3
• Gene ID (NCBI): 55616
• RRID: AB_2059021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TDY4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.