ASCC1 Polyclonal antibody

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• ASCC1 Polyclonal antibody (12301-1-AP)

ASCC1 Polyclonal Antibody for WB, ELISA

Cat No. 12301-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SMABF2, CGI18, CGI 18, ASCC 1, ASC1p50

• 

  WB analysis using 12301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12301-1-AP (ASCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12301-1-AP targets ASCC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2946

  Product name: Recombinant human ASCC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC012291

  Sequence: MEVLRPQLIRIDGRNYRKNPVQEQTYQHEEDEEDFYQGSMECADEPCDAYEVEQTPQGFRSTLRAPSLLYKHIVGKRGDTRKKIEMETKTSISIPKPGQDGEIVITGQHRNGVISARTRIDVLLDTFRRKQPFTHFLAFFLNEVEVQEGFLRFQEEVLAKCSMDHGVDSSIFQNPKKLHLTIGMLVLLSEEEIQQTCEMLQQCKEEFINDISGGKPLEVEMAGIEYMNDDPGMVDVLYAKVHMKDGSNRLQELVDRVLERFQASGLIVKEWNSVKLHATVMNTLFRKDPNAEGRYNLYTAEGKYIFKERESFDGRNILKLFENFYFGSLKLNSIHISQRFTVDSFGNYASCGQID

• Full Name: activating signal cointegrator 1 complex subunit 1
• Calculated Molecular Weight: 45.5 kDa
• Observed Molecular Weight: 45.5 kDa
• GenBank Accession Number: BC012291
• Gene Symbol: ASCC1
• Gene ID (NCBI): 51008
• RRID: AB_2059350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N9N2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASCC1 (also known as p50) is a subunit of an uncharacterized complex, the activating signal co-integrator complex (ASCC). ASCC1 has been reported as regulator fo NF-kB pathway and potently inhibits the expression of NF-kB target genes. Mutations in ASCC1 in patients with Barrett esophagus (BE) have been identified as a risk factor of esophageal adenocarcinoma (EAC).

Publications

What published studies show

ASCC1 Polyclonal antibody (Cat# 12301-1-AP) has 3 total citations, appearing in Nature Communications and Molecular Cell. The cited research spans molecular biology (DNA threading through the ASCC1 complex), cell cycle regulation (stalled ribosomes restricting progression after genotoxic stress), and innate immunity (translation stress and collided ribosomes as co-activators of cGAS). All citations used the antibody for Western Blot in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
human	WB	Mol Cell; Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.; Authors - Mark Stoneley
human	WB	Mol Cell; Translation stress and collided ribosomes are co-activators of cGAS.; Authors - Li Wan