ASCC3 Polyclonal antibody

ASCC3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 17627-1-AP

Synonyms

ASC 1 complex subunit p200, ASC1p200, ASCC3, HELIC1, Helicase, ATP binding 1, RNAH, Trip4 complex subunit p200

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Validation Data Gallery

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  WB analysis of HeLa using 17627-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17627-1-AP (ASCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17627-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17627-1-AP (ASCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17627-1-AP

  IP result of anti-ASCC3 (IP:17627-1-AP, 4ug; Detection:17627-1-AP 1:400) with HeLa cells lysate 1080ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IP: See 2 publications below

Product Information

17627-1-AP targets ASCC3 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASCC3 fusion protein Ag11848
• Full Name: activating signal cointegrator 1 complex subunit 3
• Calculated Molecular Weight: 111 aa, 13 kDa, 251 kDa
• Observed Molecular Weight: 251 kDa
• GenBank Accession Number: BC050681
• Gene Symbol: ASCC3
• Gene ID (NCBI): 10973
• RRID: AB_2059474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP,WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	WB	Mol Cell; Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.; Authors - Mark Stoneley
human	WB	Sci Rep; Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells.; Authors - Satoshi Hashimoto
mouse	WB	Cell Metab; Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response; Authors - Goda Snieckute
human	IP	EMBO J; The ASC-1 complex promotes translation initiation by scanning ribosomes; Authors - Yuki Kito

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (10-24-2023)

(Already in contact with technical support, but was asked to submit a review here as well.) Immunoblotting for ASCC3 from fresh cell extracts did not work. Included samples of ASCC3-KD (siRNA) experiment, but only unspecific bands. Tested 1:1000 and 1:500 dilution. Tested different protein extraction buffers and conditions, used fresh cells each time. Transfer of large proteins was confirmed. Sadly, for us this antibody did not work.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 and 1:500
• Cell Tissue Type: HEK293