ASCL1 Polyclonal antibody, PBS Only

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• ASCL1 Polyclonal antibody, PBS Only (31206-1-PBS)

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  WB analysis using 31206-1-AP (same clone as 31206-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31206-1-AP (ASCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31206-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31206-1-AP (same clone as 31206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31206-1-AP (ASCL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31206-1-AP (same clone as 31206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31206-1-AP (ASCL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31206-1-PBS in a different storage buffer formulation.

ASCL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31206-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Achaete-scute homolog 1, ASH1, ASH-1, bHLHa46, Class A basic helix-loop-helix protein 46

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Product Information

31206-1-PBS targets ASCL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35185

  Product name: Recombinant human ASCL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-118 aa of BC003134

  Sequence: MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPA

• Full Name: achaete-scute complex homolog 1 (Drosophila)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC003134
• Gene Symbol: ASCL1
• Gene ID (NCBI): 429
• RRID: AB_3669901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P50553
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ASCL1 (achaete-scute family bHLH transcription factor 1), also known as ASH1. It is expected to be located in nucleus. Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. The calculated molecular weight of ASCL1 is 25 kDa.