ASGR1 Recombinant monoclonal antibody

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  WB analysis using 83691-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat liver using 83691-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using 83691-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83691-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

ASGR1 Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 83691-3-RR

Clone No.240672C4

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF-P, ELISA

Synonyms

asialoglycoprotein receptor 1, HL-1, 240672C4, ASGP R 1, ASGPR

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells, human liver tissue, rat liver tissue
• Positive IF-P detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

83691-3-RR targets ASGR1 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0946

  Product name: Recombinant Human ASGR1 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: N-6*His

  Domain: 62-291 aa of NM_001671.4

  Sequence: QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL

• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: NM_001671.4
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• RRID: AB_3671295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07306-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; A High-Throughput Cell-Based Luciferase Reporter Assay for Identifying Inhibitors of ASGR1; Authors - Lingmin Gan