ASGR2 Polyclonal antibody

ASGR2 Polyclonal Antibody for WB, IHC, FC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11501-2-AP

Synonyms

CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2

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Validation Data Gallery

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  WB analysis of human liver using 11501-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 11501-2-AP

  IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug.

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  IHC staining of human liver using 11501-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 11501-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HepG2 using 11501-2-AP

  1x10^6 HepG2 cells were surface stained with 0.25 ug ASGR2 Polyclonal antibody (11501-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11501-2-AP targets ASGR2 in WB, IHC, FC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASGR2 fusion protein Ag2037
• Full Name: asialoglycoprotein receptor 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC017251
• Gene Symbol: ASGR2
• Gene ID (NCBI): 433
• RRID: AB_2059814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.