ASK1 Polyclonal antibody
ASK1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Human, Mouse and More (3)
WB, IP, IHC, ELISA
Cat no : 28201-1-AP
|Positive WB detected in||HEK-293 cells, HeLa cells, A431 cells, BxPC-3 cells|
|Positive IHC detected in||human pancreas cancer tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
28201-1-AP targets ASK1 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASK1 fusion protein Ag28092|
|Full Name||mitogen-activated protein kinase kinase kinase 5|
|Calculated molecular weight||155 kDa|
|Observed molecular weight||155 kDa|
|GenBank accession number||BC054503|
|Gene ID (NCBI)||4217|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 28201-1-AP antibody detects the 155 kDa band in SDS-PAGE.
|Product Specific Protocols|
|WB protocol for ASK1 antibody 28201-1-AP||Download protocol|
|IHC protocol for ASK1 antibody 28201-1-AP||Download protocol|
|Click here to view our Standard Protocols|
FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response
J Cell Mol Med
Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.
L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.
The IRE1 signaling pathway is involved in the protective effect of low-dose LPS on myocardial ischemia-reperfusion injury.
Toxicol Appl Pharmacol
Carnosol alleviates nonalcoholic fatty liver disease by inhibiting mitochondrial dysfunction and apoptosis through targeting of PRDX3.
Libertellenone H, a Natural Pimarane Diterpenoid, Inhibits Thioredoxin System and Induces ROS-Mediated Apoptosis in Human Pancreatic Cancer Cells.