ASK1 Polyclonal antibody

ASK1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28201-1-AP

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28201-1-AP targets ASK1 in WB, IP, IHC,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASK1 fusion protein Ag28092
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight 155 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC054503
Gene symbol MAP3K5
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 28201-1-AP antibody detects the 70 kDa isoform of ASK1 in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for ASK1 antibody 28201-1-APDownload protocol
IHC protocol for ASK1 antibody 28201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

Melatonin Alleviates Neuroinflammation and Metabolic Disorder in DSS-Induced Depression Rats.

Authors - Wei-Jie Lv
humanWB

Biomed Res Int

PICK1 Deficiency Exacerbates Sepsis-Associated Acute Kidney Injury.

Authors - Qian Dou
humanIP

J Integr Neurosci

Long noncoding RNA-regulator of reprogramming alleviates hypoxia-induced cerebral injury in mice model and human via modulating apoptosis complexes.

Authors - Qiong Zhou
humanWB

Molecules

Libertellenone H, a Natural Pimarane Diterpenoid, Inhibits Thioredoxin System and Induces ROS-Mediated Apoptosis in Human Pancreatic Cancer Cells.

Authors - Weirui Zhang
ratWB

Life Sci

The IRE1 signaling pathway is involved in the protective effect of low-dose LPS on myocardial ischemia-reperfusion injury.

Authors - Ting Wu