Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, A431 cells, BxPC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28201-1-AP targets ASK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28092 Product name: Recombinant human ASK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1180-1374 aa of BC054503 Sequence: LASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 5 |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | 155 kDa |
| GenBank Accession Number | BC054503 |
| Gene Symbol | ASK1 |
| Gene ID (NCBI) | 4217 |
| RRID | AB_2782957 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99683 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 28201-1-AP antibody detects the 155 kDa band in SDS-PAGE.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASK1 antibody 28201-1-AP | Download protocol |
| IP protocol for ASK1 antibody 28201-1-AP | Download protocol |
| WB protocol for ASK1 antibody 28201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASK1 Polyclonal antibody (Cat# 28201-1-AP) has accumulated 44 citations across journals including Redox Biology, Phytomedicine, EBioMedicine, Free Radical Biology and Medicine, FASEB Journal, Journal of Molecular and Cellular Cardiology, International Immunopharmacology, and Cancer Cell International. Associated research fields encompass cardiology, neurology, hepatology, nephrology, oncology, immunology, aging, and pharmacology, with cited applications primarily in Western blot, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Phytomedicine Dihydrocapsaicin attenuates oxidative stress and apoptosis in acute myocardial infarction via promoting Raf-1/ASK1 complex formation | ||
Phytomedicine Ginsenoside compound K alleviates brain aging by inhibiting ferroptosis through modulation of the ASK1-MKK7-JNK signaling pathway | ||
EBioMedicine USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses. | ||
Int J Biol Macromol Mycobacterium tuberculosis Rv2521 promotes ferroptosis-dependent pathogenicity by inhibiting NF-κB activation | ||
Cell Mol Gastroenterol Hepatol Sphingosine kinase 1 aggravates liver fibrosis by mediating macrophage recruitment and polarization |
