ASMTL Polyclonal antibody

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  WB analysis of human testis using 14981-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14981-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14981-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14981-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14981-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14981-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14981-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 14981-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 14981-1-AP

  Immunofluorescent analysis of A549 cells using 14981-1-AP (ASMTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ASMTL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14981-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

N-acetylserotonin O-methyltransferase-like protein, dTTPase/UTPase, dTTP/UTP pyrophosphatase, ASTML, ASMTLY

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Tested Applications

• Positive WB detected in: human testis tissue, A549 cells, HeLa cells, HL-60 cells, L02 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14981-1-AP targets ASMTL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6894

  Product name: Recombinant human ASMTL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 274-621 aa of BC002508

  Sequence: CHRTRETLPPFPTRLLELIEGFMLSKGLLTACKLKVFDLLKDEAPQKAADIASKVDASACGMERLLDICAAMGLLEKTEQGYSNTETANVYLASDGEYSLHGFIMHNNDLTWNLFTYLEFAIREGTNQHHRALGKKAEDLFQDAYYQSPETRLRFMRAMHGMTKLTACQVATAFNLSRFSSACDVGGCTGALARELAREYPRMQVTVFDLPDIIELAAHFQPPGPQAVQIHFAAGDFFRDPLPSAELYVLCRILHDWPDDKVHKLLSKVAESCKPGAGLLLVETLLDEEKRVAQRALMQSLNMLVQTEGKERSLGEYQCLLELHGFHQVQVVHLGGVLDAILATKVAP

• Full Name: acetylserotonin O-methyltransferase-like
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC002508
• Gene Symbol: ASMTL
• Gene ID (NCBI): 8623
• RRID: AB_2059989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95671
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.