ASNS Recombinant antibody

• 

  WB analysis using 85892-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85892-1-RR (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85892-1-RR

  Cells and lysate were subjected to SDS PAGE followed by western blot with 85892-1-RR (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ASNS Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85892-1-RR

Clone No.250177C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11

---

Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, K-562 cells, U2OS cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85892-1-RR targets ASNS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ASNS fusion protein Ag6393
• Full Name: asparagine synthetase
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC014621
• Gene Symbol: ASNS
• Gene ID (NCBI): 440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08243
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.