ATAD3A/B Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86461-2-RR (same clone as 86461-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

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  IF Staining of HEPG2 using 86461-2-RR (same clone as 86461-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86461-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

ATAD3A/B Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86461-2-PBS

Clone No.251342C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

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Product Information

86461-2-PBS targets ATAD3A/B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9836

  Product name: Recombinant human ATAD3B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-270 aa of BC002542

  Sequence: MSWLFGVNKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKEALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQRLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVETEARARAKAERENADIIREQIRLKASEHRQTVLESIRTAGTLFGEGFRAFVTDRDKVTATVAGLTLLAVGVYSAKNATAV

• Full Name: ATPase family, AAA domain containing 3B
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002542
• Gene Symbol: ATAD3B
• Gene ID (NCBI): 83858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T9A4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.