ATAD5 Polyclonal antibody

ATAD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24954-1-AP

Synonyms

ATAD5, C17orf41, FRAG1

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Validation Data Gallery

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  WB analysis of Jurkat using 24954-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24954-1-AP (ATAD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24954-1-AP (ATAD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24954-1-AP is ATAD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATAD5 fusion protein Ag20498
• Full Name: ATPase family, AAA domain containing 5
• Calculated Molecular Weight: 1844 aa, 208 kDa
• Observed Molecular Weight: 135-150 kDa
• GenBank Accession Number: BC015051
• Gene Symbol: ATAD5
• Gene ID (NCBI): 79915
• RRID: AB_2879819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATAD5, also named as C17orf41 and FRAG1, is involved in DNA damage response. It plays a role in PCNA deubiquitination. ATAD5 regulates the cycle of DNA replication factories. ATAD5 has two isoforms with MW 208 kDa and 135 kDa.