FITC-conjugated ATF4 Polyclonal antibody

ATF4 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

FITC Fluorescent Dye

Cat no : FITC-10835

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, FITC-10835, TAXREB67, TXREB



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

FITC-10835 targets ATF4 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF4 fusion protein Ag1279
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Calculated molecular weight 39 kDa
GenBank accession numberBC022088
Gene symbol ATF4
Gene ID (NCBI) 468
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm/520 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38kd ATF4 protein and its phosphorylated forms (50kd). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

Protocols

Product Specific Protocols
FC protocol for FITC ATF4 antibody FITC-10835Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancer Cell Int

MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin.

Authors - Yibo Dong