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ATF4 Polyclonal antibody

ATF4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10835-1-AP

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB



Tested Applications

Positive WB detected inA431 cells, DU 145 cells, HEK-293 cells, mouse kidney tissue, rat brain tissue, SGC-7901 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10835-1-AP targets ATF4 in WB, IP, IHC, IF, FC, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF4 fusion protein Ag1279
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Calculated molecular weight 39 kDa
Observed molecular weight45-50 kDa, 39 kDa
GenBank accession numberBC022088
Gene symbol ATF4
Gene ID (NCBI) 468
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

 

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

 

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

 

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

 

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

 

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

 

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

 

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)


Protocols

Product Specific Protocols
WB protocol for ATF4 antibody 10835-1-APDownload protocol
IHC protocol for ATF4 antibody 10835-1-APDownload protocol
IF protocol for ATF4 antibody 10835-1-APDownload protocol
IP protocol for ATF4 antibody 10835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress.

Authors - Yifan Jia
human,mouseWB

Br J Pharmacol

Betulinic acid alleviates endoplasmic reticulum stress-mediated NAFLD through activation of FXR in mice.

Authors - Ming Gu
mouse,humanWB

J Ophthalmol

ATF4 Involvement in TLR4 and LOX-1-Induced Host Inflammatory Response to Aspergillus fumigatus Keratitis.

Authors - Shuchao Zhang
ratWB

CNS Neurosci Ther

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

Authors - Ming-Feng Zhou
mouseWB

Cell Signal

ER stress activation impairs the expression of circadian clock and clock-controlled genes in NIH3T3 cells via an ATF4-dependent mechanism.

Authors - Lei Gao
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Natalie (Verified Customer) (12-11-2018)

Antibody worked well, although does detect some non-specific bands which appear to be dimers from the molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Rat DRG & Sciatic nerve