ATF5 Monoclonal antibody

ATF5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E5B6

Cat no : 67066-1-Ig

Synonyms

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

67066-1-Ig targets ATF5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATF5 fusion protein Ag7214
Full Name activating transcription factor 5
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC005174
Gene Symbol ATF5
Gene ID (NCBI) 22809
RRIDAB_2882376
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).

Protocols

Product Specific Protocols
WB protocol for ATF5 antibody 67066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int J Mol Sci

The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.

Authors - Meiyu Cheng
humanWB

Toxicol Lett

Bavachin induces liver injury and cell apoptosis by targeting Wnt/β-catenin/DRP1 signaling pathway mediated mitochondrial dysfunction

Authors - Ying Yang
humanWB

FEBS Open Bio

Knockdown of TMEM160 leads to an increase in reactive oxygen species generation and the induction of the mitochondrial unfolded protein response

Authors - Kosei Yamashita