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  • KD/KO Validated

ATF6 Polyclonal antibody

ATF6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (3)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 24169-1-AP

Synonyms

ATF6, ATF6 alpha, ATF6A



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human breast hyperplasia tissue, human pancreas cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24169-1-AP is ATF6 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity chicken, human, monkey, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF6 fusion protein Ag21456
Full Name activating transcription factor 6
Calculated molecular weight 75 kDa
Observed molecular weight90~100 kDa
GenBank accession numberBC014969
Gene symbol ATF6
Gene ID (NCBI) 22926
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

Protocols

Product Specific Protocols
WB protocol for ATF6 antibody 24169-1-APDownload protocol
IHC protocol for ATF6 antibody 24169-1-APDownload protocol
IF protocol for ATF6 antibody 24169-1-APDownload protocol
IP protocol for ATF6 antibody 24169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis.

Authors - Erik A Blackwood
ratWB

CNS Neurosci Ther

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

Authors - Ming-Feng Zhou
pigWB

Int J Biol Macromol

A pectic polysaccharide from water decoction of Xinjiang Lycium barbarum fruit protects against intestinal endoplasmic reticulum stress.

Authors - Chao Huang
humanWB

J Virol

Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.

Authors - Lei-Ke Zhang
  • KD Validated
humanWB

J Neuroinflammation

Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stress.

Authors - Shengchun Xu
ratWB,IF

J Ophthalmol

Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells.

Authors - Shengyu Wu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Richard (Verified Customer) (11-12-2019)

The antibody works well and gives two clean bands for cleaved and uncleaved ATF6 by western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HeLa, HEK293T