Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human cervical cancer tissue, human breast hyperplasia tissue, human pancreas cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24169-1-AP is ATF6 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat, chicken, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATF6 fusion protein Ag21456|
|Full Name||activating transcription factor 6|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC014969|
|Gene ID (NCBI)||22926|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.
|Product Specific Protocols|
|WB protocol for ATF6 antibody 24169-1-AP||Download protocol|
|IHC protocol for ATF6 antibody 24169-1-AP||Download protocol|
|IF protocol for ATF6 antibody 24169-1-AP||Download protocol|
|IP protocol for ATF6 antibody 24169-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis.
CNS Neurosci Ther
Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.
Int J Biol Macromol
A pectic polysaccharide from water decoction of Xinjiang Lycium barbarum fruit protects against intestinal endoplasmic reticulum stress.
Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.
Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stress.
Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Richard (Verified Customer) (11-12-2019)
The antibody works well and gives two clean bands for cleaved and uncleaved ATF6 by western blot.