ATG101 Polyclonal antibody

ATG101 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26562-1-AP

Synonyms

ATG101, Autophagy related protein 101, C12orf44



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:800 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26562-1-AP is ATG101 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG101 fusion protein Ag22932
Full Name chromosome 12 open reading frame 44
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC005151
Gene symbol C12orf44
Gene ID (NCBI) 60673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG101 antibody 26562-1-APDownload protocol
IHC protocol for ATG101 antibody 26562-1-APDownload protocol
IP protocol for ATG101 antibody 26562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols