ATG16L2 Polyclonal antibody, PBS Only

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• ATG16L2 Polyclonal antibody, PBS Only (24322-1-PBS)

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  WB analysis of mouse spleen using 24322-1-AP (same clone as 24322-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 24322-1-AP (same clone as 24322-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 24322-1-AP (same clone as 24322-1-PBS)

  IP result of anti-ATG16L2 (IP:24322-1-AP, 3ug; Detection:24322-1-AP 1:700) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 24322-1-AP (same clone as 24322-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 24322-1-AP (ATG16L2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

ATG16L2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 24322-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

24322-1-PBS targets ATG16L2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19399

  Product name: Recombinant human ATG16L2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 17-365 aa of BC137490

  Sequence: HIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALRVKWQEEEEGLRLVCGEMAYQVVEKGAALGTLESELQQRQSRLAALEARVAQLREARAQQAQQVEEWRAQNAVQRAAYEALRAHVGLREAALRRLQEEARDLLERLVQRKARAAAERNLRNERRERAKQARVSQELKKAAKRTVSISEGPDTLGDGMRERRETLALAPEPEPLEKEACEKWKRPFRSASATSLTLSHCVDVVKGLLDFKKRRGHSIGGAPEQRYQIIPVCVAARLPTRAQDVLDAHLSEVNAVRFGPNSSLLATGGADRLIHLWNV

• Full Name: ATG16 autophagy related 16-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 619 aa, 69 kDa
• Observed Molecular Weight: 70 kDa, 30 kDa
• GenBank Accession Number: BC137490
• Gene Symbol: ATG16L2
• Gene ID (NCBI): 89849
• RRID: AB_2879493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NAA4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.