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  • KD/KO Validated

ATG2B Polyclonal antibody

ATG2B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25155-1-AP

Synonyms

ATG2B, C14orf103



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25155-1-AP is ATG2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG2B fusion protein Ag18412
Full Name ATG2 autophagy related 2 homolog B (S. cerevisiae)
Calculated molecular weight 2078 aa, 233 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC015016
Gene symbol ATG2B
Gene ID (NCBI) 55102
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG2B antibody 25155-1-APDownload protocol
IHC protocol for ATG2B antibody 25155-1-APDownload protocol
IF protocol for ATG2B antibody 25155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

TOM40 Targets Atg2 to Mitochondria-Associated ER Membranes for Phagophore Expansion.

Authors - Zhenyuan Tang
  • KO Validated
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun
humanWB,IF

EMBO Rep

A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Authors - Mihaela Bozic
humanWB

Autophagy

Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation.

Authors - Jing-Xiang Zheng