Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16924-1-AP targets ATG4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10307 Product name: Recombinant human ATG4D protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 123-474 aa of BC068992 Sequence: TYRRDFPPLPGGCLTSDCGWGCMLRSGQMMLAQGLLLHFLPRDWTWAEGMGLGPPELSGSASPSRYHGPARWMPPRWAQGAPELEQERRHRQIVSWFADHPRAPFGLHRLVELGQSSGKKAGDWYGPSLVAHILRKAVESCSDVTRLVVYVSQDCTVYKADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPRHSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL Predict reactive species |
| Full Name | ATG4 autophagy related 4 homolog D (S. cerevisiae) |
| Calculated Molecular Weight | 474 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC068992 |
| Gene Symbol | ATG4D |
| Gene ID (NCBI) | 84971 |
| RRID | AB_2062024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86TL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.
Publications
What published studies show
ATG4D Polyclonal antibody (Cat# 16924-1-AP) has 7 citations published in Cancer Cell, Autophagy, Molecular Cell, ACS Sensors, Cell Reports, Molecular Therapy Nucleic Acids, and Frontiers in Molecular Biosciences. Research fields include autophagy regulation, glioblastoma tumorigenicity and radioresistance, LC3/GABARAP processing, non-canonical ATG8 conjugation, PM2.5-induced autophagy, mitophagy in GBM cells, and intestinal mucosa injury following subarachnoid hemorrhage.
| Species | Application | Title |
|---|---|---|
Cancer Cell MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma. | ||
Autophagy Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.
| ||
Mol Cell Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.
| ||
ACS Sens Air-Liquid Interface Microfluidic Monitoring Sensor Platform for Studying Autophagy Regulation after PM2.5 Exposure | ||
Cell Rep Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation
| ||
Mol Ther Nucleic Acids lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation. |
