ATG4D Polyclonal antibody

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  WB analysis of human brain using 16924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16924-1-AP (ATG4D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16924-1-AP (ATG4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 16924-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATG4D antibody (16924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ATG4D Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16924-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

APG4 D, APG4D, ATG, AUTL4, AUT-like 4 cysteine endopeptidase

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below

Product Information

16924-1-AP targets ATG4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG4D fusion protein Ag10307
• Full Name: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC068992
• Gene Symbol: ATG4D
• Gene ID (NCBI): 84971
• RRID: AB_2062024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TL0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Cell; MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma.; Authors - Tianzhi Huang
human	WB	Mol Cell; Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.; Authors - Joanne Durgan ; KO Validated
human,mouse	WB	ACS Sens; Air-Liquid Interface Microfluidic Monitoring Sensor Platform for Studying Autophagy Regulation after PM2.5 Exposure; Authors - Lulu Zheng
human	WB	Cell Rep; Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation; Authors - Rachel Ulferts ; KD Validated
human	WB	Mol Ther Nucleic Acids; lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation.; Authors - Changhong Liu
human	WB	Autophagy; Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.; Authors - Alexander Agrotis ; KD Validated