ATGL Polyclonal antibody

• Featured Product
• KD/KO Validated

ATGL Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

60

Reviews (2)

Cat no : 55190-1-AP

Synonyms

Adipose triglyceride lipase, PNPLA2, FP17548, EC:3.1.1.4, EC:3.1.1.3

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Validation Data Gallery

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  WB analysis of 3T3-L1 using 55190-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 55190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55190-1-AP

  WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells.

• 

  WB analysis of mouse testis using 55190-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 55190-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55190-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55190-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55190-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 55190-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 55190-1-AP

  IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, A431 cells, HeLa cells, human heart tissue, mouse heart tissue, mouse testis tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 56 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

55190-1-AP targets ATGL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: patatin-like phospholipase domain containing 2
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: NM_020376
• Gene Symbol: ATGL
• Gene ID (NCBI): 57104
• RRID: AB_11182818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

Publications

Species	Application	Title
mouse	WB	J Pineal Res; Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin.; Authors - Zhenhui Li
human		Hepatology; PMID: 29729186; Authors - Huixia Zhang
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr-/- mice; Authors - Kenan Peng
human,rat	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Li (Verified Customer) (07-12-2019)

We have tried a couple different ATGL antibodies from other vendors, but no one is working as good as we want. So we tried this one, it works great.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse muscle

FH

Shantel (Verified Customer) (05-02-2019)

Works well in Western Blot. I used mouse liver tissue, 40ug/ul protein per lane at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver

ATGL Antibody Western Blot, validation (1:1000 dilution) in mouse liver (Cat no:55190-1-AP)