ATL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16688-1-AP

Synonyms

ARL-6-interacting protein 2, ARL3IP2, ARL 6 interacting protein 2, Aip-2, Aip 2

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Validation Data Gallery

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  WB analysis using 16688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16688-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16688-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, HepG2 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Product Information

16688-1-AP targets ATL2 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATL2 fusion protein Ag10030
• Full Name: atlastin GTPase 2
• Calculated Molecular Weight: 412aa,47 kDa; 583aa,66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC053508
• Gene Symbol: ATL2
• Gene ID (NCBI): 64225
• RRID: AB_1850898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	J Cell Biol; Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.; Authors - Nan Liu
human,mouse,monkey	WB	J Cell Biol; Human atlastin-3 is a constitutive ER membrane fusion catalyst; Authors - Samantha Bryce
mouse	WB	Nature; Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy; Authors - Hector Foronda
human	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
human	WB,IF	J Biochem; Lunapark ubiquitinates atlastin-2 for the tubular network formation of the endoplasmic reticulum.; Authors - Toshiaki Sakisaka
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu