Tested Applications
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13672-1-AP targets ATP11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4654 Product name: Recombinant human ATP11B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 10-104 aa of BC033880 Sequence: SSGSAWFAIILMVVTCLFLDIIKKVFDRHLHPTSTEKAQLTETNAGIKCLDSMCCFPEGEAACASVGRMLERVIGRCSPTHISRCEISLSSLCCR Predict reactive species |
| Full Name | ATPase, class VI, type 11B |
| Calculated Molecular Weight | 1177aa, 134 kDa |
| GenBank Accession Number | BC033880 |
| Gene Symbol | ATP11B |
| Gene ID (NCBI) | 23200 |
| RRID | AB_2063150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2G3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP11B antibody 13672-1-AP | Download protocol |
| IHC protocol for ATP11B antibody 13672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP11B Polyclonal antibody (Cat# 13672-1-AP) has 3 citations published in J Immunother Cancer, Acta Neuropathol, and Life Sciences. The research spans pancreatic cancer immunity (targeting the ATP11B–PD-L1 axis), cerebral small vessel disease in a normotensive rat model, and antihypertensive mechanisms involving miRNA-150-5p in spontaneously hypertensive rats. Cited applications include WB, IHC, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Oncolytic peptide LTX-315 induces anti-pancreatic cancer immunity by targeting the ATP11B-PD-L1 axis.
| ||
Acta Neuropathol Loss of the heterogeneous expression of flippase ATP11B leads to cerebral small vessel disease in a normotensive rat model. | ||
Life Sci miRNA-150-5p associate with antihypertensive effect of epigallocatechin-3-gallate revealed by aorta miRNome analysis of spontaneously hypertensive rat. |
Reviews
What customers say
The single review for this product reports a negative experience. The user tested the antibody for Western Blot (1:1000 dilution) and Immunofluorescence (1:400 dilution) on platelets but stated they never achieved any selective staining, resulting in a low rating.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ruby (Verified Customer) (06-01-2025) | I never got any selective staining.
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